The mechanism of physiological reprogramming of cells is present in a human organism at its embryonic stage in perfect equilibrium, which is therefore able to rebuild the life cycle of a single cell. Unfortunately while the years pass, this balance gets altered and the cells decay and die due to natural or pathological causes. Now it is possible to reproduce this regeneration mechanism even in adult organisms, counteracting its decay or degeneration thanks to the growth and differentiation factors.
GROWTH AND DIFFERENTIATION FACTORS
“GROWTH AND DIFFERENTIATION FACTORS have a significant biological activity, which is essential for maintaining cellular health. Some proteins are important for the life of mitochondria, others have the function of regulating cell cycle, immunity response and so on. Therefore the biological molecules of ROE EXTRACT or CAVIAR POWDER are essential for maintaining cells in our body in optimal health state and revitalizing tissues, minimizing aging phenomena.”
It has been shown that the differentiation factors of the stem cells taken from the embryo of Zebrafish, which has more than 90% of proteins in common with the human ones, are able to differentiate the cell cycle and reprogram cells in a physiological way (without genetic manipulation).
Reversing Cancer: From cancer cells to normal cells
with a physiological therapy.
Reprogram cells with their own language
to bring them back to a state of health.
Zebrafish, or Danio rerio, is a freshwater fish which lately has become the most used animal model for laboratory studies in the world, thanks to its particular characteristics. At specific times of the growth of Zebrafish embryos, it is possible to obtain, using sophisticated techniques, an entire epigenetic code capable of reprogramming human cells and differentiating them.
HOW TO REPROGRAM IN A PHYSIOLOGICAL WAY CANCER STEM CELLS
Reprogramming of cancer stem cells, that means the possibility of conditioning the fate of tumor stem cells, offers the opportunity to findnew treatments, which act in physiological way, without genetic manipulation.
The latest scientific discoveries have highlighted and clarified the biochemical mechanisms controlling cell proliferation and differentiation. While the four transcription factors Oct4, Sox2, Klf4 and cMyc have shown to be sufficient to induce pluripotency in fibroblasts, there has in addition a lot of research studying the mechanisms of cell differentiation and the specific properties of stem cells, in particular their plasticity and capacity for trans-differentiation.
These researches have been fundamental in the field of reparative, regenerative and transplant medicine. In fact the genetic engineering production of regulatory factors identified through such research, has allowed the production of new tissues and a new category of cell therapy products, in which the main biological action is carried out by cells or tissues, even if in the presence of organic or inorganic matrices or coatings. Examples of this type of product are in vitro cultivated skin, products made of cellular elements for the reconstruction of bones, cartilages, teeth, etc. Even more importantisthe fact that a very analytical and detailed characterization of stem cells allowed the comprehension of tumor cell behaviors, that have a crucial role in determining their malignancy.
The malignant behavior of cancer cells can be attributed to the presence of cells with characteristics similar to those of stem cells. As consequence cancer research have been conceived to identify the metabolic pathways common to tumor and stem cells. This will in turn cast light on which micro-environment factors can direct these pathways towards differentiation and induce tumor cells to behave less aggressively.
Recently there has been a lot of studies which were very important to understand the role of embryonic micro-environment in controlling tumor cell behavior and to revert cancer cells towards normal phenotypes. This research is now underway, and they represent the main subject of this website.
New Paradigm in Medicine
It is important to note that research into the possibility of reprogramming cancer stem cells needs a complex approach to the issue. In fact, the problem requires the study of networks of substances and genes involved in the reprogramming phase, demanding skills in a variety of different areas of research, not simply of medical/biological, but also mathematical and modeling, in view of the complexity and non-linearity of the processes being studied.
This means to change the scientific paradigm and shift from reductionism that studies only single molecules or single mechanism to complexity, as described in the article about the new paradigm in medicine.
The future will witness our engagement in increasing numbers of scientific studies requiring a complex vision of life.
Attention to the beneficial effects of natural substances and extending the scope of attention to the entire psycho-physical organism are consistent with the aims and the mission of the medical treatment.
Conventional medicine uses chemotherapy and other treatments to kill the damaged cells.
Whereas, in this new medicine, malignant or dysfunctional cells are reprogrammed rather than killed.
Without adverse side effects.
Seven different human tumor lines (multiforma gliobastoma, melanoma, hepatocarcinoma, kidney, colon, and breast adenocarcinoma, acute lymphoblastic leukemia) have been treated with factors taken from Zebrafish embryos in four different development stages:
a) morular stage, characterized by merely multiplicatory events and therefore made of totipotent embryo stem cells,
b) medium-blastula/gastrula (50% epiboly), where the totipotent embryo stem cells differentiate into pluripotent ones,
c) five somite stage and
d) 20 somite stage, where important differentiation events, characterizing the intermediate and final embryo differentiation, take place. All cancer cell lines have shown a significant slowdown in the growth curve when treated with factors drawn during the above-mentioned cell differentiation stages, with inhibition percentages ranging from 73% of the glioblastoma and 26% of the melanoma.
Objective tumor response median overall survival and performance status have been evaluated. Results showed a 19.8% patients who experienced a regression and 16% patients who experienced a stabilization with an overall survival of more than 60% of responsive patients after 40 months, against 10% of non responsive ones. A wide improvement of performance status has been registered in a great majority of patients (82.6%).
Pier Mario Biava, an occupational doctor, graduated in Medicine at the University of Pavia, specializing first in occupational medicine at the University of Padua and later in hygiene at the University of Trieste. He has been studying the relationship between cancer and cell differentiation for several years: he has isolated stem growth and differentiation factors that can inhibit or slow down the growth of various types of human tumors and, in general, direct the fate of normal and pathological stem cells. This is of a significant importance in the field of prevention and treatment of various types of diseases such as chronic degenerative diseases, as well as in the field of regenerative medicine in renewal and regeneration of tissues. This research can also have positive effects in the field of integrative treatments of stem cell transplants and, in the future, may even replace them.
Having been a Professor at the School of Specialization in Occupational Medicine in Trieste for many years, he currently works at the Institute of Research and Treatment Multimedica of Milan.
He is the author of over 100 scientific publications and several books such as: ” L’Aggressione Nascosta – Limiti Sanitari di Esposizione ai Rischi ” published by Feltrinelli, ” Complessità e Biologia” published by Bruno Mondadori and ” Il Cancro e la Ricerca del Senso Perduto” published by Springer,” Il Senso Ritrovato “edited by Ervin Laszlo and published by Springer and “Dal segno al simbolo – The manifesto of the new paradigm in medicine” written with Diego Frigoli and Ervin Laszlo and published by Persiani.
He also takes part in the Scientific Committees of several international journals in the field of oncology and epidemiology.
Scientific responsible for formation of the occupational health service in South America founded in Quito from 1984 to 1990
Member of the WWF Italia Scientific Committee from 1986 to present (still in office), Vice President WWF Italy 2006 -2009
Member of the CDA Unicredit Foundation from 2002 to 2005 where he leaded planning of all health cooperation interventions in Africa.
Dr. Pier Mario Biava and his team of scientists have shown that specific GROWTH and DIFFERENTIATION FACTORS can increase the life span of cells, preventing the cutting of telomeres and contributing to the synthesis of proteins, which stop the aging of cells.
Dr. Pier Mario Biava on Telomeres:
“Telomeres get shorter each time the cells divide. When the telomere becomes too short, the chromosome reaches a critical length and the cell can no longer replicate. This means that the cell ages and dies. However, GROWTH and DIFFERENTIATION FACTORS can be a very effective tool to promote cellular senescence antagonists in a physiological way. The result is a healthy cell and potentially an inversion of the aging process”
TELOMERS are essential parts of human cells that influence the way we age. Telomeres represent sort of a “cap” of DNA that protects cells from errors during their replication. Over time this hood tends to shorten and lose its protective properties. Thanks to the factors of Zebrafish this problem can be prevented by preserving telomers.
are only of differentiation, thus of physiological programming
EACH STAGE HAS ITS BIOLOGICAL FUNCTION
Different Starting Points
Cure to Cancer
THE WORLD IS NOT A MECHANICAL AGGREGATE,
BUT AN ORGANIC HOLISTIC UNITY"
LASZLO: " Biava and I had a very different starting points.
Biava started from trying to find a cure to cancer,
and I from trying to see what fundamental insights
underline current findings in the sciences.
At the end we reached the same conclusions:
BIAVA: "Cells communicate with one another
by using codes of meaning:
this is true in a literal and not in a metaphorical sense"
Without adverse side effects
The Growth Factors appropriately matched to the differentiation factors prevent cellular senescence and therefore aging
In this documentary you will find many answers both on the nature of mechanisms that mediate the transfer of epigenetic information to DNA: the differentiation factors and on the practical applications of these researches.